R&D Engineer (internship)
08-2016 - 01-2017 Diseño/Ingeniería civil/Ingeniería industrial Optimisation and development of a transient expression platform for difficult to produce his-tagged proteins.
Acquired skills:
- Suspension cell culture (HEK 293 cells)
- Production of recombinant proteins
- Protein purification at bench and using the AKTA chromatography systems (affinity chromatography)
- Protein characterization: SDS-Page, SE-HPLC, Octet QK Device, ELISA
- Design of experiments (DoE) using JMP 12.0
Results: An effective and easy-to-apply method has been developed allowing the production of proteins with 2-fold increase in purity.